3D Confocal Imaging of Pollen

Confocal Imaging of 3D Network Structures

High-resolution Confocal Imaging of the Blood-brain Barrier: Imaging, 3D Reconstruction, and Quantification of Transcytosis

The blood-brain barrier (BBB) is a dynamic multicellular interface that regulates the transport of molecules between the circulation and the brain. Transcytosis across the BBB regulates the delivery of hormones, metabolites, and therapeutic antibodies to the brain parenchyma. Here, we present a protocol that combines immunofluorescence of free-floating sections with laser scanning confocal micr...

Hemispherical Confocal Imaging

We propose a new imaging method called hemispherical confocal imaging to visualize clearly a particular depth in a 3-D scene. The key optical component is a turtleback reflector which is a specially designed polyhedral mirror. To synthesize a hemispherical aperture, we combined the turtleback reflector with a coaxial camera and projector, to create on a hemisphere many virtual cameras and proje...

Monitoring the 3D nanostructures of bulk heterojunction polymer solar cells using confocal lifetime imaging.

In this study, the exciton lifetime images within the photoactive layers of poly(3-hexylthiophene) (P3HT) and [6,6]-phenyl-C61-butyric acid methyl ester (PCBM) are revealed by confocal optical microscopy combined with the fluorescence module. The images reveal that the active layers during slow solvent evaporation provide 3D pathways for charge transport and the concentration gradient through t...

QTIP: Quantifier for Topographic Index of Presynaptic-terminal using 3D Confocal Imaging

Topography mapping is an important and general strategy for the nervous system to encode the locations of sensory stimuli. It has been difficult to analyze the relative spatial position between adjacent neurons in the same nervous system because of complex fine branches of axon terminals, especially at single-cell resolution. This paper describes an automatic image analysis tool QTIP (Quantifie...